Biotin, also known as vitamin H, but is more commonly called Biotin. It is a vitamin which is B-complex in nature and is water soluble. Biotin helps in transferring carbon dioxide along with helping in various metabolic reactions. A steady blood sugar level can also be maintained by the help of biotin. Hair and nails are strengthened by the consumption of biotin. Therefore, a lot of hair and cosmetic products use biotin as one of its ingredients. The body’s daily requirement of vitamin B7 is produced by the intestinal bacteria. This therefore reduces the chances of vitamin B7 deficiency. There a large number of foods that contain biotin in large quantities. Swiss chard, tomatoes, liver,eggs, almonds, cumcuber, cauliflower, legume, soybeans, raspberries, carrots are some of those foods.

Chromium is a natural trace mineral in human nutrition and is needed for proper metabolism of sugar in humans. It is vital for metabolism of fats and carbohydrates. It helps move blood sugar from the bloodstream into cells to be utilized as energy and turn fats, carbohydrates, and proteins into energy. Chromium helps stimulate fatty acid and cholesterol synthesis, which are needed for brain function and other body processes. It activates numerous enzymes that are required to drive many chemical reactions essential to life. Chromium supplements also improve glucose tolerance in people with Turner's syndrome, a disease associated with glucose intolerance. It is also vital for insulin metabolism. It regulates hunger and decreases cravings.

L-Isoleucine- The second of three branched chain amino acids it is important in blood sugar regulation and producing hemoglobin. After exercise, it is responsible for muscle recovery and important in the metabolism to produce energy.

L-Leucine is useful in conjunction with L-Valine and L-Isoleucine as an amino acid supplement. L-Leucine is an amino acid that a body cannot produce on its own. It is expected to be received externally from food sources such as brown rice and nuts or even through some of the dietary supplementation. L-Leucine is showing numerous advantages in the athletic qualities. Within six week of training with L-Leucine in association, a person is able to maximize the aerobic power. In the six weeks the strength and power in the upper body increases. However, research and studies is limited carried on the athletic performance. L-Leucine incorporated into the muscle protein in continuation with two weeks exercise regime will give adequate resistance and the muscle protein synthesis will certainly show an increase. L-Leucine is a preservative of the muscles, but is as well a supplement that enhances the capabilities of muscle building owing to the enhanced protein synthesis rate.

L-Valine- L-Valine is one the three Branched Chain Amino Acids (BCAAs). The other two BCAAs are L-isoleuine and L-leucine. L-Valine is required by the body for the proper function of the nervous system and the brain. L-Valine is not processed by the liver; instead, it goes directly to the muscles. It helps enhance muscle growth and help it to recover faster after an intense workout. It also helps in muscle tissue repair and maintains the proper amount of nitrogen in the body. It can also be used as an energy source that gives energy and strength to the muscles. L-Valine has other benefits; it is also helpful in liver regeneration, improvement of insomnia and nervousness, and is an effective appetite suppressant.

Vitamin B12 helps maintain different forms of cells in the human body. For example, practices such as formation, maintenance and repair of red blood cells largely depend on Vitamin B12. It also takes care of the nerve cells in the body, helping maintain a strong nervous system. It is a key entity for regular formation of DNA in the body. In case of its deficiency in the body, abnormal cell formation, called megaloblasts, occur, which can cause anemia in the body. It is a good source of relieving the body from fatigue and weakness. Cyanocobalamin also helps reduce the cholesterol levels in the body. It is efficacious in treatment of Alzheimer's disease.

Vitamin B6, which active form pyridoxal phosphate or otherwise known as PLP and pyridoxal-5’-phosphate, is a prosthetic group of tightly bound cofactors responsible for many reactions within the amino acid metabolism and the manipulation of the enzymatic reaction behind the release of glucose from glycogen. Vitamin B6 generally comprises three organic compounds, pyridoxine, pyridoxal and pyridoxamine, all of them heterocyclic organic compounds that are converted in the human body to the active pyridoxal phosphate form. There are three reactions attributed to PLP, namely transamination, alpha elimination and beta elimination. Over one hundred and forty enzyme activities depend on pyridoxal phosphate, neurotransmitter and histamine synthesis, synthesis and function of hemoglobin, and gene expression. As a cofactor, PLP is active in amino acid metabolism, glucogenesis and lipid metabolism.

Zinc is acknowledged as essential to the human organism. It is a trace element found in over a hundred and up to three hundred enzymes. It is the second most important mineral in the body, after iron. Zinc is also defined as an important catalytic agent, which is active in enzymatic reactions such as hydroxilation, which is essential for the protein forming amino acid proline, but also for hypoxia response. Zinc is naturally found in red meat, milk, spinach, nuts, oysters, beans and oats. Low zinc counts can attribute to a weakened immune system, interfere with muscle building, cause diarrhea and all kind of other deficiencies. In body building circles, Zinc is an important supplement, valued for the indirect increment of testosterone and the muscle building role.